|
| Status |
Public on Dec 15, 2011 |
| Title |
Laccaria/Populus deltoides ECM greenhouse-1 |
| Sample type |
RNA |
| |
|
| Source name |
three-month old Laccaria/Populus ectomycorrizal tissue
|
| Organisms |
Populus deltoides; Laccaria bicolor S238N-H82 |
| Characteristics |
tissue: L. bicolor/P.deltoides ectomycorrhizae were synthesized by growing cuttings of Populus deltoides for three month in pots containing Terragreen (Brenntag Lorraine, Toul, France) mixed with fungal inoculum in a peat-vermiculite mix (4:1,v/v). Mycorrhizal root tips were harvested and immediately frozen in liquid nitrogen.
age: 3 months
|
| Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was carried out using the RNeasy Plant Mini Kit (Qiagen). Total RNA
preparations were amplified using the SMART PCR cDNA Synthesis Kit (Clontech)
according to the manufacturer’s instructions
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company
|
| Scan protocol |
Scanning by NimbleGen Company
|
| Data processing |
Expression values reflect across-array quantile normalization and gene calls
generated using the Robust Multichip Average (RMA). For information on RMA see
Biostatistics 2003 Apr; 4(2): 249-264
|
| |
|
| Submission date |
Oct 03, 2011 |
| Last update date |
Dec 15, 2011 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL14641 |
| Series (1) |
| GSE32559 |
Laccaria bicolor gene expression in different tissues and with different host trees |
|
