|
| Status |
Public on Oct 20, 2016 |
| Title |
Saliva Sample from control individual #2 |
| Sample type |
RNA |
| |
|
| Source name |
Saliva Sample from control individual
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: control
individual: individual 2
biomaterial: saliva
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MiRNA profiling of seven saliva samples (three from patients with TSCC, four from healthy control individuals) were performed using Agilent 8X60K Human V19 miRNA microarray comprised of 2006 probes including control probes from Sanger miRBase (Release 19). All related reagents were purchased from Agilent Inc.
|
| Label |
cy3
|
| Label protocol |
100 ng of total RNA from each sample were labeled with Cy3 using “Agilent miRNA Labeling and Hyb Kit” according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The labeled RNA samples were heat denatured and hybridized to microarray slide and incubated at 55°C for 20 hours.
|
| Scan protocol |
After hybridization, microarray slides were washed and scanned in Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Description |
K2
|
| Data processing |
Feature Extraction v10.7.3.1 (Agilent Technologies, CA) software was utilized to extract all features of the scanned image. Raw data were normalized by quantile normalization using Bioconductor software with R version 2.15.
|
| |
|
| Submission date |
May 18, 2015 |
| Last update date |
Oct 21, 2016 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL18044 |
| Series (1) |
| GSE69002 |
MicroRNA Expression Profiling of Saliva in Tongue Squamous Cell Carcinoma Patients |
|
