|
| Status |
Public on Oct 20, 2016 |
| Title |
MicroRNA Expression Profiling of Saliva in Tongue Squamous Cell Carcinoma Patients |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
41% of all oral carcinomas have been found to localize at tongue, where they were characterized as being aggressive and having capacity to locally invade and relapse frequently. Despite considerable enhancements in the cancer diagnosis and treatment techniques, tongue squamous cell carcinoma (TSCC) still remains to be one of the most common and lethal cancer types in the head and neck region. In this study, we aimed to identify a signature of saliva-specific microRNAs (miRNAs) expression in TSCC patients. To explore putative diagnostic biomarkers, we compared the miRNA profiles of saliva samples obtained from 3 TSCC patients and 4 healthy control individual using Agilent miRNA microarray (V19). Three of the differentially expressed miRNAs were selected for further validation with quantitative reverse-transcription PCR (qRT-PCR) using saliva samples of 25 TSCC patients and 25 healthy control individuals. Microarray analysis demonstrated that 428 miRNA probes were deregulated in TSCC patients when compared to control group and qRT-PCR results validated the reduced expression of miR-139-5p in TSCC saliva. Further analysis of post-operative saliva samples of TSCC patients revealed that miR-139-5p level elevated to normal level after surgery, pointing its diagnostic and prognostic power. As a conclusion, we propose saliva as a feasible source in routine patient examination for early diagnosis of TSCC patients, and our results suggest saliva miR-139-5p as a novel potential diagnostic marker.
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| Overall design |
We compared the miRNA profiles of saliva samples obtained from 3 TSCC patients and 4 healthy control individual using Agilent miRNA microarray (V19).
Two group comparison
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| Contributor(s) |
Creighton C, Ozen M, Zhang Y |
| Citation(s) |
26650483 |
| Submission date |
May 18, 2015 |
| Last update date |
Dec 13, 2017 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18044 |
Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Feature Number version) |
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| Samples (7)
|
| GSM1689913 |
Saliva Sample from (Tongue Cancer) TCa patient #1 |
| GSM1689914 |
Saliva Sample from (Tongue Cancer) TCa patient #2 |
| GSM1689915 |
Saliva Sample from (Tongue Cancer) TCa patient #3 |
| GSM1689916 |
Saliva Sample from control individual #1 |
| GSM1689917 |
Saliva Sample from control individual #2 |
| GSM1689918 |
Saliva Sample from control individual #3 |
| GSM1689919 |
Saliva Sample from control individual #4 |
|
| Relations |
| BioProject |
PRJNA284274 |
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