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Series GSE88929 Query DataSets for GSE88929
Status Public on Mar 29, 2017
Title Epigenetic signatures of gestational diabetes mellitus on ATP5A1, PRKCH, SLC17A4 and HIF3A cord blood methylation
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Background: Intrauterine exposure to gestational diabetes mellitus (GDM) confers a lifelong increased risk for metabolic and other complex disorders to the offspring. GDM-induced epigenetic modifications modulating gene regulation and persisting into later life are generally assumed to mediate these increased disease risks. To identify candidate genes for fetal programming, we compared genome-wide methylation patterns of fetal cord bloods (FCBs) from GDM and control pregnancies.
Methods and Results: Using Illumina's 450K methylation arrays and following correction for multiple testing, 65 CpG sites (52 of which are associated with genes) displayed significant methylation differences between GDM and control samples. Three of four candidate genes, ATP5A1, PRKCH, and SLC17A4, from our methylation screen and one, HIF3A, from the literature were validated by bisulfite pyrosequencing. The GDM effect on FCB methylation was more pronounced in women with insulin-dependent GDM who had a more severe metabolic phenotype than women with dietetically treated GDM. However, the effect remained significant after adjustment for the maternal BMI and gestational week in a multivariate regression model.
Conclusions: Our study supports an association between maternal GDM and the epigenetic status of the exposed offspring. Consistent with a multifactorial disease model, the observed FCB methylation changes are of small effect size but affect multiple genes/loci. The identified genes are primary candidates for transmitting GDM effects to the next generation. They also may provide useful biomarkers for the diagnosis and prognosis of adverse prenatal exposures and assessing the success of interventions during pregnancy.
 
Overall design Bisulphite converted DNA from the 38 samples were hybridised to the Illumina Infinium 450K Human Methylation Beadchip

Please note that the experiments were carried out as two batches (GSM2355316-GSM2355353 and GSM2355354-GSM2355447), and they were normalized and filtered independently.
 
Contributor(s) Haaf T
Citation(s) 28360945
Submission date Oct 19, 2016
Last update date Mar 22, 2019
Contact name Marcus Dittrich
Organization name University of Würzburg
Department Biozentrum
Street address Biozentrum, Am Hubland
City Würzburg
ZIP/Postal code 97074
Country Germany
 
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (132)
GSM2355316 genomic DNA from umbilical cord blood 1
GSM2355317 genomic DNA from umbilical cord blood 2
GSM2355318 genomic DNA from umbilical cord blood 3
Relations
BioProject PRJNA349175

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE88929_RAW.tar 183.1 Mb (http)(custom) TAR
GSE88929_non_normalized_GSM2355316-GSM2355353.txt.gz 77.9 Mb (ftp)(http) TXT
GSE88929_non_normalized_GSM2355354-GSM2355447.txt.gz 185.9 Mb (ftp)(http) TXT
Processed data included within Sample table
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