|
| Status |
Public on Mar 29, 2017 |
| Title |
genomic DNA from umbilical cord blood 116 |
| Sample type |
genomic |
| |
|
| Source name |
DGDM_umbilical cord blood
|
| Organism |
Homo sapiens |
| Characteristics |
sample group: DGDM
maternal age (yr): 36
mode of birth: Cesarean section
gestational week: 38
birth weight (g): 2500
gender: female
blood ph: 7.26
maternal bmi: 21
maternal height (cm): 160
weight before pregnancy (kg): 54
weight before birth(kg): 65
hba1c: 5,20
nicotine consumption: Non_Smoker
placenta weight: 373,00
|
| Treatment protocol |
After collection it was immediately frozen at -80°C until further use
|
| Growth protocol |
Umbilical cord blood from newborns was collected by obstetricians
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified using Qiagen FlexiGene DNA Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450K Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
SAMPLE 78
|
| Data processing |
BeadStudio software v3.2, Normalized (dasen and BMIQ) Beta values; the non_normalized.txt contains Unmethylated and methylated signal intensities.
|
| |
|
| Submission date |
Oct 19, 2016 |
| Last update date |
Mar 29, 2017 |
| Contact name |
Marcus Dittrich |
| Organization name |
University of Würzburg
|
| Department |
Biozentrum
|
| Street address |
Biozentrum, Am Hubland
|
| City |
Würzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE88929 |
Epigenetic signatures of gestational diabetes mellitus on ATP5A1, PRKCH, SLC17A4 and HIF3A cord blood methylation |
|
