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| Status |
Public on Jan 01, 2016 |
| Title |
Genome-wide profiling of PARP1 reveals an interplay with gene regulatory regions and DNA methylation |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
To address the global impact of PARP-1 on DNA methylation, we treated cells with PJ34 (PARylation inhibitor) and isolated genomic DNA from vehicle and PJ34 treated cells. This DNA was bisulfite treated and hybridized to the Illumina infinium Methylation 450 Beadchip. We next used these RNA-seq data sets (control, PARP-1 KD and PARylation inhibited) to assess whether PARP plays a role in DNA methylation by assessing differential methylation patterns.
PARP1 mediates methylation patterns.
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| Overall design |
DNA from vehicle and PJ34 (PARylation inhibited) cells. 750ng of geneomic DNA was bisulfite converted and used for the Illumia infinium HD methylation assay.
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| Contributor(s) |
Nalabothula N, Taha A, Shao X, Fondufe-Mittendorf Y |
| Citation(s) |
26305327 |
| Submission date |
Sep 25, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Yvonne Nsokika Fondufe-Mittendorf |
| E-mail(s) |
y.fondufe-mittendorf@uky.edu
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| Phone |
8593230091
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| Organization name |
University of Kentucky
|
| Department |
Biochemistry
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| Lab |
Mittendorf
|
| Street address |
741 South Limestone dr.
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| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536-0509 |
| Country |
USA |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA262012 |
