|
| Status |
Public on Jan 01, 2016 |
| Title |
DNA-methylation in PJ-34 treated cells |
| Sample type |
genomic |
| |
|
| Source name |
Breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
agent: PJ34
|
| Treatment protocol |
MCF7 cells were then treated with vehicle and PJ34 for 2hours respectively.
|
| Growth protocol |
Cell cultures were incubated at 37 degree with humidified air and 5% CO2. Cells were harvested after each treatment. Cells were grown ensuring that 95% of the cells were viable. Harvested cells were centrifuged for five minutes at 200 X g.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Qiagen Dneasykit according to manusfacturers instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to the Illumina Infinium Human Methylation450 Beadchip using the standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using the HiScan with the standard recommended settings
|
| Description |
Sample name: MCF7-PJ34
|
| Data processing |
GenomeStudio v.2011.1
|
| |
|
| Submission date |
Sep 25, 2014 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Yvonne Nsokika Fondufe-Mittendorf |
| E-mail(s) |
y.fondufe-mittendorf@uky.edu
|
| Phone |
8593230091
|
| Organization name |
University of Kentucky
|
| Department |
Biochemistry
|
| Lab |
Mittendorf
|
| Street address |
741 South Limestone dr.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536-0509 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE61744 |
Genome-wide profiling of PARP1 reveals an interplay with gene regulatory regions and DNA methylation |
|
