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| Status |
Public on May 16, 2014 |
| Title |
Hormonal Regulation of MicroRNA Expression in Rat Adrenal Gland |
| Platform organism |
synthetic construct |
| Sample organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine miRNA profiles in rat adrenals from animals treated with vehicle, ACTH, 17α-ethinyl estradiol (17α-E2) or dexamethasone (DEX). miRNA expression profiling was performed using Affymetrix® GeneChip® miRNA 2.0 Arrays. Together with qRT-PCR, several adrenal miRNAs have been identified, whose expression is subject to regulation by one or more than one hormone.
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| Overall design |
RNA was extracted from four group rat adrenal gland and miRNA profiles were established using microarray using Affymetrix® GeneChip® miRNA 2.0 Arrays and confirmed with qRT-PCR. The microarray data were analyzed using Partek® Genome Suite software, version 6.3 Copyright© 2008 (Partek Inc., St. Louis, MO, USA) to comprehensively evaluate the differential expression level of the various samples.
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| Contributor(s) |
Hu Z, Shen W, Cortez Y, Tang X, Liu L, Kraemer FB, Azhar S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 21, 2013 |
| Last update date |
May 02, 2017 |
| Contact name |
Wen-Jun Shen |
| E-mail(s) |
wenjun@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Medicine
|
| Lab |
Kraemer
|
| Street address |
3801 Miranda Avenue
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA203707 |
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