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Status |
Public on May 16, 2014 |
Title |
Rat adrenal CON-1 |
Sample type |
RNA |
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|
Source name |
Rat adrenal with vehicle
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Organism |
Rattus norvegicus |
Characteristics |
gender: Male tissue: Adrenal gland strain: Sprague Dawley age: 3 monthes weight: 225-250g
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Treatment protocol |
Subsequently rats were randomly divided into Four groups (3 rats in each group): 1) non-stressed controls (C), 2) rats treated with Cortrosyn (ACTH) (10 IU) every 24h for 4 days, with the last injection on day 4 given 1h prior to harvesting of adrenal tissues, 3) rats treated with 17α-ethinyl estradiol (17α-E2), 10 mg/kg BW sc every 24h for 5 days, and 4) rats treated with dexamethasone (DEX), a single injection of 100 ug, sc for a 24 h period. At the end of treatment, animals were euthanized and adrenal tissues collected for various molecular and biochemical measurements.
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Growth protocol |
Three monthes male Sprague Dawley (SD) rats were purchased from Harlan Laboratories (Indianapolis, IN). They were allowed to acclimatize to a new controlled environment (25 ± 2oC, 55 ± 5% relative humidity with a 12h light-dark cycle) for approximately one week.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purified with miRNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
500 ng total RNA containing low molecular weight RNA was labled with FlashTag™ Biotin HSR RNA Labeling Kit according to the standard Affymetrix protocol.
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Hybridization protocol |
Affymetrix® GeneChip® Hybridization, Wash and Stain Kit was used for the hybridization according the standard protocal. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G. The measurements were carried out by the Protein and Nucleic Acid (PAN) Microarray Facility of Stanford University.
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Description |
miRNA expression data from rat adrenal gland treated with phosphate buffered saline (PBS).
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Data processing |
The microarray data were analyzed using Partek® Genome Suite software, version 6.3 Copyright© 2008 (Partek Inc., St. Louis, MO, USA)
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Submission date |
May 21, 2013 |
Last update date |
May 16, 2014 |
Contact name |
Wen-Jun Shen |
E-mail(s) |
wenjun@stanford.edu
|
Organization name |
Stanford University
|
Department |
Medicine
|
Lab |
Kraemer
|
Street address |
3801 Miranda Avenue
|
City |
Palo Alto |
State/province |
CA |
ZIP/Postal code |
94304 |
Country |
USA |
|
|
Platform ID |
GPL14613 |
Series (1) |
GSE47131 |
Hormonal Regulation of MicroRNA Expression in Rat Adrenal Gland |
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