|
| Status |
Public on May 16, 2014 |
| Title |
Rat adrenal 17-1 |
| Sample type |
RNA |
| |
|
| Source name |
Rat adrenal with 17α-E2
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Male
tissue: Adrenal gland
strain: Sprague Dawley
age: 3 monthes
weight: 225-250g
|
| Treatment protocol |
Subsequently rats were randomly divided into Four groups (3 rats in each group): 1) non-stressed controls (C), 2) rats treated with Cortrosyn (ACTH) (10 IU) every 24h for 4 days, with the last injection on day 4 given 1h prior to harvesting of adrenal tissues, 3) rats treated with 17α-ethinyl estradiol (17α-E2), 10 mg/kg BW sc every 24h for 5 days, and 4) rats treated with dexamethasone (DEX), a single injection of 100 ug, sc for a 24 h period. At the end of treatment, animals were euthanized and adrenal tissues collected for various molecular and biochemical measurements.
|
| Growth protocol |
Three monthes male Sprague Dawley (SD) rats were purchased from Harlan Laboratories (Indianapolis, IN). They were allowed to acclimatize to a new controlled environment (25 ± 2oC, 55 ± 5% relative humidity with a 12h light-dark cycle) for approximately one week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified with miRNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
500 ng total RNA containing low molecular weight RNA was labled with FlashTag™ Biotin HSR RNA Labeling Kit according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Affymetrix® GeneChip® Hybridization, Wash and Stain Kit was used for the hybridization according the standard protocal. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G. The measurements were carried out by the Protein and Nucleic Acid (PAN) Microarray Facility of Stanford University.
|
| Description |
miRNA expression data from rat adrenal gland treated with 17α-E2.
|
| Data processing |
The microarray data were analyzed using Partek® Genome Suite software, version 6.3 Copyright© 2008 (Partek Inc., St. Louis, MO, USA)
|
| |
|
| Submission date |
May 21, 2013 |
| Last update date |
May 16, 2014 |
| Contact name |
Wen-Jun Shen |
| E-mail(s) |
wenjun@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Medicine
|
| Lab |
Kraemer
|
| Street address |
3801 Miranda Avenue
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE47131 |
Hormonal Regulation of MicroRNA Expression in Rat Adrenal Gland |
|
