|
Status |
Public on Sep 06, 2012 |
Title |
TscWT1_blood |
Sample type |
RNA |
|
|
Source name |
Tsc2 wildtype mouse, blood
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: Tsc2 wildtype tissue: blood gender: Female
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
A total of 250 ng was processed using established Affymetrix protocols for the generation of biotin-labeled cRNA.
|
|
|
Hybridization protocol |
The cRNA was quantified (using A260) and fragmented. Fragmented cRNA was hybridized on the GeneChips according to the manufacturer's standard protocol.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000 at 2.5 μm resolution.
|
Description |
Tsc2 wildtype mouse 1, blood
|
Data processing |
The data were analyzed with Probe Logarithmic Intensity Error (PLIER) estimation method using Affymetrix Power Tools.
|
|
|
Submission date |
Sep 05, 2012 |
Last update date |
Sep 06, 2012 |
Contact name |
Sek Won Kong |
E-mail(s) |
swkong@enders.tch.harvard.edu
|
Phone |
617-919-2689
|
Organization name |
Boston Children's Hospital
|
Department |
Informatics Program
|
Lab |
EN137
|
Street address |
300 Longwood Avenue
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE40630 |
Divergent dysregulation of gene expression in Fmr1 and Tsc2 knockout mouse models of autism spectrum disorders |
|