|
| Status |
Public on Sep 06, 2012 |
| Title |
FmrKO2_brain |
| Sample type |
RNA |
| |
|
| Source name |
Fmr1 knockout mouse, cerebellum
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Fmr1 knockout
tissue: cerebellum
gender: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
A total of 250 ng was processed using established Affymetrix protocols for the generation of biotin-labeled cRNA.
|
| |
|
| Hybridization protocol |
The cRNA was quantified (using A260) and fragmented. Fragmented cRNA was hybridized on the GeneChips according to the manufacturer's standard protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000 at 2.5 μm resolution.
|
| Description |
Fmr1 knockout mouse 2, cerebellum
|
| Data processing |
The data were analyzed with Probe Logarithmic Intensity Error (PLIER) estimation method using Affymetrix Power Tools.
|
| |
|
| Submission date |
Sep 05, 2012 |
| Last update date |
Sep 06, 2012 |
| Contact name |
Sek Won Kong |
| E-mail(s) |
swkong@enders.tch.harvard.edu
|
| Phone |
617-919-2689
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Lab |
EN137
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE40630 |
Divergent dysregulation of gene expression in Fmr1 and Tsc2 knockout mouse models of autism spectrum disorders |
|
