|
| Status |
Public on Apr 01, 2014 |
| Title |
IDC_D30_tumoral sample |
| Sample type |
genomic |
| |
|
| Source name |
IDC tumoral sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast cancer
tissue subtype: Invasive Ductal Carcinoma (IDC)
tumor grade: 2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from tumor fragments using a standard phenol/chloroform procedure. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 to generate .CEL files for downstream analysis.
|
| Description |
Hybridized to SNP6.0
|
| Data processing |
CEL files and TXT files (generated by Affymetrix Genotyping Console 3.0.1) were processed by GAP (Popova T et al, Genome Biol, 2009) and Partek® GS software version 6.5 build 6.10.1020
|
| |
|
| Submission date |
Apr 04, 2012 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Jaydutt D Bhalshankar |
| E-mail(s) |
jaydutt.bhalshankar@curie.fr
|
| Phone |
0330156246517
|
| Organization name |
Institut CURIE
|
| Department |
INSERM UNIT 830
|
| Lab |
Genetics and Biology of Cancer
|
| Street address |
26 rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75248 |
| Country |
France |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE37035 |
Polarity gene alterations in pure invasive micropapillary carcinomas of the breast |
|
