|
Status |
Public on Apr 01, 2014 |
Title |
IDC_D19_tumoral sample |
Sample type |
genomic |
|
|
Source name |
IDC tumoral sample
|
Organism |
Homo sapiens |
Characteristics |
tissue: breast cancer tissue subtype: Invasive Ductal Carcinoma (IDC) tumor grade: 2
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted from tumor fragments using a standard phenol/chloroform procedure. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
Label |
biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
Scan protocol |
The arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 to generate .CEL files for downstream analysis.
|
Description |
Hybridized to SNP6.0
|
Data processing |
CEL files and TXT files (generated by Affymetrix Genotyping Console 3.0.1) were processed by GAP (Popova T et al, Genome Biol, 2009) and PartekĀ® GS software version 6.5 build 6.10.1020
|
|
|
Submission date |
Apr 04, 2012 |
Last update date |
Apr 01, 2014 |
Contact name |
Jaydutt D Bhalshankar |
E-mail(s) |
jaydutt.bhalshankar@curie.fr
|
Phone |
0330156246517
|
Organization name |
Institut CURIE
|
Department |
INSERM UNIT 830
|
Lab |
Genetics and Biology of Cancer
|
Street address |
26 rue d'Ulm
|
City |
Paris |
ZIP/Postal code |
75248 |
Country |
France |
|
|
Platform ID |
GPL6801 |
Series (1) |
GSE37035 |
Polarity gene alterations in pure invasive micropapillary carcinomas of the breast |
|