|
| Status |
Public on Nov 08, 2012 |
| Title |
liver, Wt females_4966 |
| Sample type |
RNA |
| |
|
| Source name |
Wt female liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: female
genotype/variation: wild type
tissue: liver
|
| Treatment protocol |
Livers were flash-frozen in liquid nitrogen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Qiagen RNA was extracted according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using instructions from the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized for 16h at 45°C to gene chips of the MOE430A series for each category of pooled RNA: 4953,4954,4955,4956. Biotinylated cRNA was hybridized for 16 h at 45°C to gene chips of the MOE430B series.
|
| Scan protocol |
Samples were scanned with the Affymetrix Gene Chip Scanner 3000
|
| Description |
Wt females_4966
|
| Data processing |
The Affymetrix Gene Chip Operating Software and the MAS 5 algorithm were used for processing. The .txt file outputs are linear and scaled to an array-mean of 1500.
|
| |
|
| Submission date |
Mar 13, 2012 |
| Last update date |
Nov 09, 2012 |
| Contact name |
Nancy Marcus |
| E-mail(s) |
nmarcus@slu.edu
|
| Organization name |
St. Louis University
|
| Department |
Pediatrics
|
| Lab |
Teckman
|
| Street address |
1100 S. Grand,Doisy Research Center
|
| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63104 |
| Country |
USA |
| |
|
| Platform ID |
GPL340 |
| Series (1) |
| GSE36478 |
Gene Expression Levels in PiZ mice Compared to Wild-type (Wt)C57Bl/6 |
|
