|
| Status |
Public on Feb 01, 2024 |
| Title |
Amosite-induced rat mesothelioma, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
amosite asbestos-induced malignant peritoneal mesothelioma
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
tissue: malignant peritoneal sarcomatoid mesothelioma
|
| Treatment protocol |
Malignant mesotheliomas were induced in Wistar rats by single peritoneal injection with MWCNTs and amosite asbestos suspended in artificial lung medium.
|
| Growth protocol |
Tumors were collected from study animals. About half of the material was flash-frozen and stored in -80°C, while the remaining material was subjected to histopathological and immunohistochemical analyses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from tumors and control peritoneal tissues was isolated using the ‘AllPrep DNA/RNA Mini Kit’ (Qiagen), following manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
The synthesis of double-stranded cDNA, the in vitro transcription, the cleanup of aRNA and biotin-labeling were undertaken with the 3´IVT Plus Kit (Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
Biotinylated fragmented aRNA (10 µg ) was hybridized onto the GeneChip® Rat Genome 230 2.0 array. Hybridization was performed for 16 h at 60 rpm and 45°C in the GeneChip® Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from amosite asbestos-induced malignant peritoneal mesothelioma
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC 4.0), using Affymetrix default settings, QC metrics, and PLIER normalization.
|
| |
|
| Submission date |
May 31, 2023 |
| Last update date |
Feb 01, 2024 |
| Contact name |
Stella Marie Reamon-Buettner |
| E-mail(s) |
stella.reamon-buettner@item.fraunhofer.de
|
| Phone |
+49 511 5350 527
|
| Organization name |
Fraunhofer-ITEM
|
| Department |
Preclinical Pharmacology & In Vitro Toxicology
|
| Street address |
Nikolai-Fuchs Strasse1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE233803 |
Transcriptome profiles of malignant peritoneal mesotheliomas of rats induced by multiwalled carbon nanotubes (MWCNTs) and amosite asbestos |
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