|
Status |
Public on Aug 03, 2011 |
Title |
MM control shRNA- biological replicate 2 |
Sample type |
RNA |
|
|
Source name |
HeLa cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa treatment: mismatch MM control
|
Treatment protocol |
HeLa cells were transduced with shRNA virus against Cyclin T2 or Cyclin T1 or MM control for five days.
|
Growth protocol |
HeLa cells were maintained in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (Invitrogen)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using the Qiagen Rneasy kit according to the manufacturer's protocol and RNA quality was determined using an Aligent 2100 Bioanalyzer
|
Label |
Biotin
|
Label protocol |
standard Affymetrix protocol
|
|
|
Hybridization protocol |
standard Affymetrix protocol
|
Scan protocol |
Affymetrix Gene Chip Scanner 3100
|
Data processing |
CEL files for each array were uploaded into GeneSifter and the data normalized and log transformed using the GC-RMA algorithm.
|
|
|
Submission date |
Apr 02, 2011 |
Last update date |
Aug 03, 2011 |
Contact name |
Andrew P Rice |
E-mail(s) |
arice@bcm.edu
|
Phone |
713-798-5774
|
Fax |
713-798-3490
|
Organization name |
Baylor College of Medicine
|
Department |
Molecular Virology & Microbiology
|
Street address |
MS BCM385,One Baylor Plaza
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE28339 |
Gene expression data following Cyclin T2 and Cyclin T1 depletion by shRNA in HeLa cells |
|