NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE28339 Query DataSets for GSE28339
Status Public on Aug 03, 2011
Title Gene expression data following Cyclin T2 and Cyclin T1 depletion by shRNA in HeLa cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We have characterized gene expression changes in HeLa cells following long term depletion of Cyclin T2 or Cyclin T1 using shRNA
 
Overall design HeLa cells were transduced with VSV-G pseudotyped lentiviral vectors expressing shRNA against either Cyclin T2 or Cyclin T1. As a control, cells were transduced with shRNA vector with four nucleotides mismatch in the Cyclin T1 mRNA that has been previously shown to have minimal effects on mRNA expression levels. The vectors have a GFP reporter that can be used to estimate transduction efficiency. Five days post-transduction, cells were harvested and total RNA extracted. Transcriptional profiling was carried out on these RNA samples. Two independent biological replicate experiments were carried out in this analysis and the xpression values normalized by GC-RMA and averaged. The following comparisons were made: MM to Cyclin T2 and MM to Cyclin T1.
 
Contributor(s) Rice AP, Ramakrishnan R
Citation(s) 21791050
Submission date Apr 02, 2011
Last update date Mar 25, 2019
Contact name Andrew P Rice
E-mail(s) arice@bcm.edu
Phone 713-798-5774
Fax 713-798-3490
Organization name Baylor College of Medicine
Department Molecular Virology & Microbiology
Street address MS BCM385,One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM700769 MM control shRNA- biological replicate 1
GSM700770 Cyclin T1 shRNA-biological replicate 1
GSM700771 Cyclin T2 shRNA-biological replicate 1
Relations
BioProject PRJNA139469

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28339_RAW.tar 47.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap