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Status |
Public on Aug 03, 2011 |
Title |
Gene expression data following Cyclin T2 and Cyclin T1 depletion by shRNA in HeLa cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We have characterized gene expression changes in HeLa cells following long term depletion of Cyclin T2 or Cyclin T1 using shRNA
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Overall design |
HeLa cells were transduced with VSV-G pseudotyped lentiviral vectors expressing shRNA against either Cyclin T2 or Cyclin T1. As a control, cells were transduced with shRNA vector with four nucleotides mismatch in the Cyclin T1 mRNA that has been previously shown to have minimal effects on mRNA expression levels. The vectors have a GFP reporter that can be used to estimate transduction efficiency. Five days post-transduction, cells were harvested and total RNA extracted. Transcriptional profiling was carried out on these RNA samples. Two independent biological replicate experiments were carried out in this analysis and the xpression values normalized by GC-RMA and averaged. The following comparisons were made: MM to Cyclin T2 and MM to Cyclin T1.
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Contributor(s) |
Rice AP, Ramakrishnan R |
Citation(s) |
21791050 |
Submission date |
Apr 02, 2011 |
Last update date |
Mar 25, 2019 |
Contact name |
Andrew P Rice |
E-mail(s) |
arice@bcm.edu
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Phone |
713-798-5774
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Fax |
713-798-3490
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Organization name |
Baylor College of Medicine
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Department |
Molecular Virology & Microbiology
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Street address |
MS BCM385,One Baylor Plaza
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (6)
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GSM700769 |
MM control shRNA- biological replicate 1 |
GSM700770 |
Cyclin T1 shRNA-biological replicate 1 |
GSM700771 |
Cyclin T2 shRNA-biological replicate 1 |
GSM700772 |
MM control shRNA- biological replicate 2 |
GSM700773 |
Cyclin T1 shRNA-biological replicate 2 |
GSM700774 |
Cyclin T2 shRNA-biological replicate 2 |
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Relations |
BioProject |
PRJNA139469 |