|
| Status |
Public on Aug 03, 2011 |
| Title |
Cyclin T1 shRNA-biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
treatment: Cyclin T1 knockdown
|
| Treatment protocol |
HeLa cells were transduced with shRNA virus against Cyclin T2 or Cyclin T1 or MM control for five days.
|
| Growth protocol |
HeLa cells were maintained in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (Invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Qiagen Rneasy kit according to the manufacturer's protocol and RNA quality was determined using an Aligent 2100 Bioanalyzer
|
| Label |
Biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3100
|
| Data processing |
CEL files for each array were uploaded into GeneSifter and the data normalized and log transformed using the GC-RMA algorithm.
|
| |
|
| Submission date |
Apr 02, 2011 |
| Last update date |
Aug 03, 2011 |
| Contact name |
Andrew P Rice |
| E-mail(s) |
arice@bcm.edu
|
| Phone |
713-798-5774
|
| Fax |
713-798-3490
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular Virology & Microbiology
|
| Street address |
MS BCM385,One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28339 |
Gene expression data following Cyclin T2 and Cyclin T1 depletion by shRNA in HeLa cells |
|
