|
| Status |
Public on Dec 01, 2011 |
| Title |
APM_48h_1 |
| Sample type |
RNA |
| |
|
| Source name |
embryos treated with APM for 48 h (2-50 hpf)
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: embryo
exposure time: 48 h
treatment: APM 5,83 µM
|
| Treatment protocol |
50 embryos per treatment, treatments: control, 5,83 µM azinphos-methyl (APM), 509 µM 1,4-dimethoxybenzene (DMB), 14,2 µM 2,4-dinitrophenol (DNP), 24 h and 48 h (26-50 and 2-50 hpf), respectively; all in 4 independent replicates
|
| Growth protocol |
Zebrafish embryos were collected directly after fertilisation and treated starting 2 hpf in exposure medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Synthesis of cDNA, cRNA and cRNA-labeling was performed with the Agilent Low RNA Input Linear Amplification Kit according to the manufacturer’s instructions. cRNA was labelled with Cy3.
|
| |
|
| Hybridization protocol |
Hybridization was performed using the Agilent Gene Expression Hybridization Kit following manufacturer's instructions.
|
| Scan protocol |
Scanned on an Agilent scanner. Images were quantified using Agilent Feature Extraction Software (version 9.1.1.1).
|
| Description |
Gene expression of embryos treated with APM for 48 h (2-50 hpf)
|
| Data processing |
Feature extraction files (processed signals) were used for statistics, quantile normalisation of all processed signals before significance testing.
|
| |
|
| Submission date |
Mar 04, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Wibke Busch |
| E-mail(s) |
wibke.busch@ufz.de
|
| Phone |
00493412351515
|
| Organization name |
UFZ Helmholtz Centre for Environmental Research
|
| Department |
Bioanalytical Ecotoxicology
|
| Lab |
Scholz
|
| Street address |
Permoser Str. 15
|
| City |
Leipzig |
| ZIP/Postal code |
04318 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6563 |
| Series (1) |
| GSE27680 |
Transcriptional Response of Zebrafish Embryos Exposed to Neurotoxic Compounds Reveals a Muscle Activity Dependent hspb11 Expression |
|
