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Status |
Public on Dec 01, 2011 |
Title |
DMB_24h_4 |
Sample type |
RNA |
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|
Source name |
embryos treated with DMB for 24 h (26-50 hpf)
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Organism |
Danio rerio |
Characteristics |
developmental stage: embryo exposure time: 24 h treatment: DMB 509 µM
|
Treatment protocol |
50 embryos per treatment, treatments: control, 5,83 µM azinphos-methyl (APM), 509 µM 1,4-dimethoxybenzene (DMB), 14,2 µM 2,4-dinitrophenol (DNP), 24 h and 48 h (26-50 and 2-50 hpf), respectively; all in 4 independent replicates
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Growth protocol |
Zebrafish embryos were collected directly after fertilisation and treated starting 2 hpf in exposure medium.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Synthesis of cDNA, cRNA and cRNA-labeling was performed with the Agilent Low RNA Input Linear Amplification Kit according to the manufacturer’s instructions. cRNA was labelled with Cy3.
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Hybridization protocol |
Hybridization was performed using the Agilent Gene Expression Hybridization Kit following manufacturer's instructions.
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Scan protocol |
Scanned on an Agilent scanner. Images were quantified using Agilent Feature Extraction Software (version 9.1.1.1).
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Description |
Gene expression of embryos treated with DMB for 24 h (26-50 hpf)
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Data processing |
Feature extraction files (processed signals) were used for statistics, quantile normalisation of all processed signals before significance testing.
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|
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Submission date |
Mar 04, 2011 |
Last update date |
Dec 01, 2011 |
Contact name |
Wibke Busch |
E-mail(s) |
wibke.busch@ufz.de
|
Phone |
00493412351515
|
Organization name |
UFZ Helmholtz Centre for Environmental Research
|
Department |
Bioanalytical Ecotoxicology
|
Lab |
Scholz
|
Street address |
Permoser Str. 15
|
City |
Leipzig |
ZIP/Postal code |
04318 |
Country |
Germany |
|
|
Platform ID |
GPL6563 |
Series (1) |
GSE27680 |
Transcriptional Response of Zebrafish Embryos Exposed to Neurotoxic Compounds Reveals a Muscle Activity Dependent hspb11 Expression |
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