|
| Status |
Public on Nov 18, 2022 |
| Title |
TSPC cells, NAC，7days 3 |
| Sample type |
SRA |
| |
|
| Source name |
tendon
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: tendon
cell line: mesenchymal stem cells
cell type: tendon stem/progenitor cells
genotype: tenogenic differentiation
treatment: F12+NAC(500uM)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchwork (parameter- Quality limit: 0.05)
Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork (parameters- mismath cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8)
Read count extraction and normalization were performed using CLC genomic benchwork
Assembly: mm10
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Nov 15, 2022 |
| Last update date |
Nov 18, 2022 |
| Contact name |
Kang Lu |
| E-mail(s) |
lukang306656@hospital.cqmu.edu.cn
|
| Phone |
18223936353
|
| Organization name |
The Second Affiliated Hospital of Chongqing Medical University
|
| Street address |
Gaotanyanzheng street
|
| City |
Chongqing |
| ZIP/Postal code |
400030 |
| Country |
China |
| |
|
| Platform ID |
GPL18694 |
| Series (1) |
| GSE218038 |
N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling |
|
| Relations |
| BioSample |
SAMN31742703 |
| SRA |
SRX18275408 |
