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| Status |
Public on Mar 02, 2023 |
| Title |
PVN_HT3 [miRNA] |
| Sample type |
SRA |
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| Source name |
Brain
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| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Brain
treatment: Hepatectomy
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissue samples of the PVN and CEA, and the preparation of the cDNA library and the sequencing were performed by Novo Gene Biotech Co. Ltd. (Beijing, China).
A strand-specific library strategy was used to construct the mRNA and circRNA libraries. Sequencing data for the miRNA library were generated using a NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA).
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
PVN_HT3
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| Data processing |
The insert size of the library was examined using an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and only effective concentrations >2 nM were used for sequencing.
The raw reads were filtered to obtain the clean reads after removing adapter-related reads, reads containing uncertain bases, and low-quality reads with base quality phred score ≤ 20.
The clean reads were then mapped to the human reference genome (GRCh38/HG38) using HISAT2 algorithms and were quantitatively analyzed using the featureCounts software.
Assembly: hg38
Supplementary files format and content: tab-delimited text files include readcount values for each Sample
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| Submission date |
Sep 27, 2022 |
| Last update date |
Mar 02, 2023 |
| Contact name |
Wei Hu |
| E-mail(s) |
hw15301050259@gmail.com
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| Phone |
+17717429024
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| Organization name |
Fudan university
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| Street address |
Dongan Road 130
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| City |
Shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
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| Platform ID |
GPL18694 |
| Series (2) |
| GSE214277 |
Electroacupuncture Alleviates Perioperative Hypothalamus-Pituitary-Adrenal Axis Dysfunction via CircRNA-miRNA-mRNA Networks [miRNA] |
| GSE214279 |
Electroacupuncture Alleviates Perioperative Hypothalamus-Pituitary-Adrenal Axis Dysfunction via CircRNA-miRNA-mRNA Networks |
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| Supplementary data files not provided |
| Processed data are available on Series record |
| Raw data not provided for this record |
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