|
| Status |
Public on Oct 05, 2011 |
| Title |
12W35-2 |
| Sample type |
RNA |
| |
|
| Source name |
Crown
|
| Organism |
Triticum aestivum |
| Characteristics |
genotype/variation: winter Manitou
developmental stage: seedling (3-leaf stage)
biorep: 2
stress: low temperature
time (duration of cold-treatment): 35 days
|
| Treatment protocol |
Wheat seedlings at 3-leaf stage were cold treated at 6 degree centigrade for 0, 2, 14, 28, 35, 42, 56 and 70 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using TRIZOL following the protocol recommended by the manufacturer
|
| Label |
Biotin
|
| Label protocol |
The RNA was labelled with biotin according to standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Microarray slides were hybridized to Biotin-labelled RNA using Affymetrix standard protocol
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix
|
| Description |
SAMPLE 14
|
| Data processing |
The data were analyzed with GeneSpring GX using RMA as normalization method.
|
| |
|
| Submission date |
Aug 31, 2010 |
| Last update date |
Oct 05, 2011 |
| Contact name |
Debbie Laudencia-Chingcuanco |
| Organization name |
USDA-ARS
|
| Department |
Western Regional Research Center
|
| Lab |
Genomics and Gene Discovery Research Unit
|
| Street address |
800 Buchanan Street
|
| City |
Albany |
| State/province |
CA |
| ZIP/Postal code |
94710 |
| Country |
USA |
| |
|
| Platform ID |
GPL3802 |
| Series (1) |
| GSE23889 |
Transcriptome profiling and expression analyses of genes critical to wheat adaptation to low temperature |
|
