|
Status |
Public on Oct 05, 2011 |
Title |
12W14-1 |
Sample type |
RNA |
|
|
Source name |
Crown
|
Organism |
Triticum aestivum |
Characteristics |
genotype/variation: winter Manitou developmental stage: seedling (3-leaf stage) biorep: 1 stress: low temperature time (duration of cold-treatment): 14 days
|
Treatment protocol |
Wheat seedlings at 3-leaf stage were cold treated at 6 degree centigrade for 0, 2, 14, 28, 35, 42, 56 and 70 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using TRIZOL following the protocol recommended by the manufacturer
|
Label |
Biotin
|
Label protocol |
The RNA was labelled with biotin according to standard Affymetrix protocol
|
|
|
Hybridization protocol |
Microarray slides were hybridized to Biotin-labelled RNA using Affymetrix standard protocol
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix
|
Description |
SAMPLE 7
|
Data processing |
The data were analyzed with GeneSpring GX using RMA as normalization method.
|
|
|
Submission date |
Aug 31, 2010 |
Last update date |
Oct 05, 2011 |
Contact name |
Debbie Laudencia-Chingcuanco |
Organization name |
USDA-ARS
|
Department |
Western Regional Research Center
|
Lab |
Genomics and Gene Discovery Research Unit
|
Street address |
800 Buchanan Street
|
City |
Albany |
State/province |
CA |
ZIP/Postal code |
94710 |
Country |
USA |
|
|
Platform ID |
GPL3802 |
Series (1) |
GSE23889 |
Transcriptome profiling and expression analyses of genes critical to wheat adaptation to low temperature |
|