|
| Status |
Public on Apr 21, 2021 |
| Title |
RNA pull-down using Oligo Pool B2 |
| Sample type |
SRA |
| |
|
| Source name |
GH4C1 cell line
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: GH4C1
genotype: wild type
treatment: Psoralen
|
| Treatment protocol |
Cells were crosslinked with psoralen
|
| Growth protocol |
GH4C1 somatolactotroph pituitary cells (ATCC_ CCL-82.2™, Molsheim, France) were grown in HamF10 medium supplemented with 15% horse serum and 2% fetal calf serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using NucleoSpin®RNA (Macherey-Nagel).
RNA libraries were prepared for sequencing using standard Illumina protocols; RNA-Seq 2 x 150 bp Strand-specific
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Cell crosslinked using psoralen
RNA pull-down using Oligo Pool B (Files B)
|
| Data processing |
Library strategy: RNA pulldown
STAR
FeatureCount
Genome_build: Rnor6.0
Supplementary_files_format_and_content: abundance measurement (counts)
|
| |
|
| Submission date |
Oct 25, 2020 |
| Last update date |
Apr 21, 2021 |
| Contact name |
Anne-Marie François-Bellan |
| E-mail(s) |
anne-marie.francois@univ-amu.fr
|
| Organization name |
CNRS-AMU
|
| Department |
Facute de Medecine
|
| Lab |
INP UMR 7051 CNRS-AMU
|
| Street address |
27 Bd Jean Moulin
|
| City |
Marseille |
| ZIP/Postal code |
13005 |
| Country |
France |
| |
|
| Platform ID |
GPL18694 |
| Series (1) |
| GSE160069 |
Direct RNA-RNA interaction between Neat1 and RNA targets, as a mechanism for RNAs paraspeckle retention |
|
| Relations |
| BioSample |
SAMN16547570 |
| SRA |
SRX9358354 |
