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| Status |
Public on Apr 21, 2021 |
| Title |
Direct RNA-RNA interaction between Neat1 and RNA targets, as a mechanism for RNAs paraspeckle retention |
| Organism |
Rattus norvegicus |
| Experiment type |
Other
|
| Summary |
The goal of this study was to determine if the lncRNA Neat1, in addition to its structural role, may also be directly involved in the binding by base pairing of RNAs and consequently in their retention in paraspeckles
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| Overall design |
GH4C1 sommatolactotroph pituitary cells (ATCC_ CCL-82.2™, Molsheim, France) were grown in HamF10 medium supplemented with 15% horse serum and 2% fetal calf serum. Cells were crosslinked with psoralen which only fix the RNA-RNA interactions. We then perfomed an RNA pull-down and RNAseq to determine the RNAs direcltely linked to Neat1
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| Contributor(s) |
Jacq A, Becquet D, Guillen S, Boyer B, Goutierrez M, Franc J, François-Bellan A |
| Citation(s) |
33573434 |
| Submission date |
Oct 25, 2020 |
| Last update date |
Apr 22, 2021 |
| Contact name |
Anne-Marie François-Bellan |
| E-mail(s) |
anne-marie.francois@univ-amu.fr
|
| Organization name |
CNRS-AMU
|
| Department |
Facute de Medecine
|
| Lab |
INP UMR 7051 CNRS-AMU
|
| Street address |
27 Bd Jean Moulin
|
| City |
Marseille |
| ZIP/Postal code |
13005 |
| Country |
France |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA671727 |
| SRA |
SRP288507 |
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