|
| Status |
Public on Oct 12, 2010 |
| Title |
adipose_tissue_calorie-restriction_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
adipose_tissue, 2week calorie-restriction after 8week over-feeding
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Visceral adipose tissue
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Visceral adipose tissued were stained by nile red and were collected by surgical manipulation under a fluorescence microscope. These tissue were stored in RNA-later (Ambion). Total RNA were extracted using RNeasy Mini Kit (Qiagen) and were quantified using a spectrophotometer (NanoDrop ND-100)
|
| Label |
Cy3
|
| Label protocol |
Three hundred nanograms of total RNA was converted into labeled cRNA using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent) and Cy3-labeled cRNA (1.5 micrograms) were prepared according to manufacture’s protocol
|
| |
|
| Hybridization protocol |
1.5 ug of Cy3-labelled cRNA was fragmented following the manufacturers instructions. On completion of the fragmentation reaction, 250 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Zebrafish Whole Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by air spray
|
| Scan protocol |
The hybridized microarrays were scanned using a scanner (Agilent G2565BA)
|
| Description |
Gene expression after 2week calorie-restriction in obesity model zebrafish
|
| Data processing |
The scanned images were analyzed with Feature Extraction software (Agilent). Bad probes were removed based on gIsFeatNonUnifOL,gIsPosAndSignif and gIsWellAboveBG
|
| |
|
| Submission date |
Oct 14, 2009 |
| Last update date |
Oct 14, 2010 |
| Contact name |
oka takehiko |
| Organization name |
mie university
|
| Department |
Graduate School of Medicine
|
| Lab |
Department of Molecular and Cellular Pharmacology
|
| Street address |
2-174 Edobashi
|
| City |
Tsu |
| State/province |
Mie |
| ZIP/Postal code |
514-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6563 |
| Series (1) |
| GSE18566 |
Gene expression analysis of diet induced obesity model zebrafish |
|
