|
| Status |
Public on Nov 19, 2019 |
| Title |
RB vs. RN Replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
resistant seedling pool infested with BPH for 24h
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: seedling
age: 10 days
phenotype: BPH-resistant
treatment: infested with BPH
|
| Treatment protocol |
At the second-leaf stage, the seedlings were infested with 2nd to 3rd instar BPH nymphs at 10 insects per seedling.
|
| Growth protocol |
RH, 02428 and two rice pools (consisting of ten highly resistant or susceptible plants from an F2 population derived from a cross between the BPH-resistant rice cultivar Rathu Heenati (RH) and the susceptible cultivar 02428) were used for cDNA microarray assays. To ensure all seedlings were at the same growth stage for BPH infestation, seeds were pre-germinated in petri dishes. Seeds were sown in a 10 cm diameter plastic pot with a hole at the bottom.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Trizol and were purified with QIAGEN RNeasy Mini Kit following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Two-color microarray analysis was performed with the Rice Expression 4x44K Microarray
|
| |
|
| Channel 2 |
| Source name |
resistant seedling pool without BPH infestation
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: seedling
age: 10 days
phenotype: BPH-resistant
treatment: without BPH infestation
|
| Treatment protocol |
At the second-leaf stage, the seedlings were infested with 2nd to 3rd instar BPH nymphs at 10 insects per seedling.
|
| Growth protocol |
RH, 02428 and two rice pools (consisting of ten highly resistant or susceptible plants from an F2 population derived from a cross between the BPH-resistant rice cultivar Rathu Heenati (RH) and the susceptible cultivar 02428) were used for cDNA microarray assays. To ensure all seedlings were at the same growth stage for BPH infestation, seeds were pre-germinated in petri dishes. Seeds were sown in a 10 cm diameter plastic pot with a hole at the bottom.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Trizol and were purified with QIAGEN RNeasy Mini Kit following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Two-color microarray analysis was performed with the Rice Expression 4x44K Microarray
|
| |
|
| |
| Hybridization protocol |
Each Cy5-labelled( 875 ng cRNA) was mixed with each of Cy3-labelled( 875 ng cRNA). The mixtures were applied to microarrays and hybridization was performed at 65°C for 17 h. After hybridization, slides were washed sequential
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software(version 10.5.1.1)
|
| Description |
Biological replicate 2 of 3
|
| Data processing |
Agilent Feature Extraction Software (version 10.5.1.1) with default setting was used for background subtraction and LOWESS normalization
|
| |
|
| Submission date |
Nov 18, 2019 |
| Last update date |
Nov 19, 2019 |
| Contact name |
JUN HE |
| Organization name |
Nanjing Agricultural University
|
| Street address |
Weigang 1
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210095 |
| Country |
China |
| |
|
| Platform ID |
GPL8852 |
| Series (1) |
| GSE140562 |
Transcriptome analysis of BPH-resistant and BPH-susceptible rice seedlings in response to BPH infestation |
|
