|
| Status |
Public on Apr 30, 2024 |
| Title |
WP-0614 treated C |
| Sample type |
SRA |
| |
|
| Source name |
WP-0614
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: melanoma cancer cell line (MBM)
cell line: WP-0614
treatment: icaritin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the ZR-Duet DNA/RNA MiniPrep kit (Zymo Research, Irvine CA.)
mRNA libraries were constructed using the Bioo Scientific NEXTflex Rapid Directional RNA-seq Library Prep Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
Base calling and demultiplexing were processed using CASAVA version 1.8
Reads were mapped using the GENCODE release 19 reference using STAR version 2.4.2a
Read counts were generated using the quantMode GeneCounts option in STAR.
|
| |
|
| Submission date |
May 09, 2019 |
| Last update date |
Apr 30, 2024 |
| Contact name |
Dave S.B. Hoon |
| E-mail(s) |
hoond@jwci.org
|
| Organization name |
Saint John's Cancer Institute
|
| Department |
Translational Molecular Medicine
|
| Street address |
2200 Santa Monica Boulevard
|
| City |
Santa Monica |
| State/province |
CA |
| ZIP/Postal code |
90403 |
| Country |
USA |
| |
|
| Platform ID |
GPL21697 |
| Series (1) |
| GSE130956 |
Investigation of ST8SIA1 expression in icaritin-treated and untreated melanoma cell lines |
|
| Relations |
| BioSample |
SAMN11609573 |
| SRA |
SRX5811149 |
