|
| Status |
Public on Sep 15, 2020 |
| Title |
FL5_FDC |
| Sample type |
RNA |
| |
|
| Source name |
Follicular lymphoma primary sample from lymph node
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: cocultured with FDC
|
| Treatment protocol |
FL and FL-FDC cultures were treated with 500nM idelalisib for 48h
|
| Growth protocol |
FL primary samples were seed onto preestablised layesr of FDC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was obtained by using the TRIzol reagent followed by a cleaning step with the RNAeasy kit. RNA integrity was examined with the Agilent 2100 Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA
|
| |
|
| Hybridization protocol |
RNA samples were processed to Affymetrix GeneChip HT HG-U219 arrays.
|
| Scan protocol |
Scanning was processed in the Gene Titan instrument.
|
| Description |
Gene expression data control cells
|
| Data processing |
Determination of the signal value for each probe set of the array were obtained with GeneChip® Command Console® Software (AGCC) (Affymetrix).
|
| |
|
| Submission date |
May 08, 2019 |
| Last update date |
Sep 15, 2020 |
| Contact name |
Patricia Pérez-Galán |
| E-mail(s) |
pperez@clinic.cat
|
| Phone |
932275400
|
| Organization name |
IDIBAPS
|
| Department |
Hemato-oncology
|
| Street address |
Centre Esther Koplowitz, Hemato-oncology dept, Rosello 149-153, 2nd floor
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE130918 |
Effect of Idelalisib in follicular lymphoma monocultures and cocultured with follicular dendritic cells (FDC) |
|
