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Status |
Public on Jan 01, 2009 |
Title |
DRIP 13_muscle taken at slaughter, mean end weight of 110 kg |
Sample type |
RNA |
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Source name |
Muscle Longissimus dorsi, DRIP 13
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Organism |
Sus scrofa |
Characteristics |
F2 animals of a resource population based on the breeds Duroc and Pietrain
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
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Description |
Gene expression data from Muscle Longissimus dorsi sample of F2 animals
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Data processing |
Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500.
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Submission date |
Jan 18, 2008 |
Last update date |
Jan 22, 2008 |
Contact name |
Klaus Wimmers |
E-mail(s) |
s.wimmers@fbn-dummerstorf.de
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Phone |
0049 3820868700
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Organization name |
Leibniz Institute for Farm Animal Biology (FBN)
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Street address |
Wilhelm-Stahl-Allee 2
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City |
Dummerstorf |
ZIP/Postal code |
18196 |
Country |
Germany |
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Platform ID |
GPL3533 |
Series (1) |
GSE10204 |
Gene expression data from Muscle Longissimus dorsi sample of F2 animals |
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