|
| Status |
Public on Jul 29, 2016 |
| Title |
9 |
| Sample type |
RNA |
| |
|
| Source name |
AORTA
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6
gender: male
genotype: MR-knockout
age: aged
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total aortic miRNA was extracted from 3 or 12 month old mice using QIAzol, followed by column purification.
|
| Label |
biotin
|
| Label protocol |
standard protocol
|
| |
|
| Hybridization protocol |
Reverse-transcribed aortic miRNA was hybridized to Mouse miRNA2.0 microarrays (Affymetrix, Santa Clara, CA) at the Boston University Medical School Center for Genetics and Genomics.
|
| Scan protocol |
standard protocol
|
| Description |
aortic miRNA expression data
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the affy package included within in the Bioconductor software suite (version 2.10.0). Affymetrix probeset mapping (also obtained from Bioconductor version 2.10.0) was used to normalize the miRNA 2.0 microarray. Differential miRNA expression was assessed with the limma package by creating linear models or using the empirical Bayes (moderated) t test. Correction for multiple hypothesis testing was accomplished using the Benjamini-Hochberg false discovery rate (FDR). All microarray analyses were performed using the R environment for statistical computing (version 2.12.0).
|
| |
|
| Submission date |
Jul 28, 2016 |
| Last update date |
Jul 29, 2016 |
| Contact name |
Iris Jaffe |
| E-mail(s) |
ijaffe@tuftsmedicalcenter.org
|
| Organization name |
Tufts Medical Center
|
| Street address |
800 Washington Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE84960 |
Smooth muscle cell mineralocorticoid receptor regulation of vascular microRNAs with aging |
|
