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Sample GSM21707

Query DataSets for GSM21707

Status

Public on Apr 17, 2004

Title

Normal basal cell - 20mJ

Sample type

RNA

Source name

Human skin; normal

Organism

Homo sapiens

Extracted molecule

total RNA

Description

This data set was generated as part of Biogen Community Lab's science fair mentorship program. In brief, human basal cells (ATCC: CRL-7761, designation TE 353.Sk) were cultured to 80% confluence. After removing media, cells were treated with a titration of ultraviolet light (Biorad GS GeneLinker, 254 nm energy maximum). Media was then added and cells were grown for 24 hours. Total RNA was harvested using RLT buffer and Qiagen RNeasy kit. 5 ug of total RNA was labeled using standard Affymetrix protocols for Eukaryotic target preparation (GeneChip Expression Analysis Technical Manual Revision 4).
Keywords = UV
Keywords = ultraviolet
Keywords = basal cell

Submission date

Apr 15, 2004

Last update date

May 27, 2005

Contact name

Jeff Shearstone

E-mail(s)

jshearstone@acetylon.com

Phone

617-245-1321

Organization name

Acetylon Pharmaceuticals

Department

Biology

Street address

70 Fargo Street

City

Boston

State/province

ZIP/Postal code

02210

Country

USA

Platform ID

GPL96

Series (1)

GSE1317

Ultraviolet induced gene expression in human basal cell culture

Data table header descriptions
ID_REF
VALUE	Affymetrix GCOS intensity
ABS_CALL	The call by Affymetrix GCOS in an intensity analysis that indicates if the transcript was present (P) or absent (A)
DETECTION_P_VALUE	P-value from Affymetrix GCOS that indicates the significance level of the intensity value

Data table
ID_REF	VALUE	ABS_CALL	DETECTION_P_VALUE
AFFX-BioB-5_at	2196.8	P	0.002275
AFFX-BioB-M_at	3843.9	P	0.00007
AFFX-BioB-3_at	2143.3	P	0.000225
AFFX-BioC-5_at	6362.8	P	0.000297
AFFX-BioC-3_at	6847.8	P	0.00007
AFFX-BioDn-5_at	6473	P	0.00006
AFFX-BioDn-3_at	45159.9	P	0.00007
AFFX-CreX-5_at	72954.9	P	0.000044
AFFX-CreX-3_at	108812.8	P	0.000044
AFFX-DapX-5_at	113.8	A	0.262827
AFFX-DapX-M_at	144.8	A	0.58862
AFFX-DapX-3_at	32.1	A	0.876428
AFFX-LysX-5_at	38.6	A	0.617411
AFFX-LysX-M_at	109.1	A	0.749204
AFFX-LysX-3_at	36.2	A	0.544587
AFFX-PheX-5_at	25.6	A	0.843268
AFFX-PheX-M_at	10.3	A	0.9273
AFFX-PheX-3_at	147.6	A	0.51489
AFFX-ThrX-5_at	80.5	A	0.262827
AFFX-ThrX-M_at	24.4	A	0.921998

Total number of rows: 22283

Table truncated, full table size 605 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM21707.CEL.gz	3.5 Mb	(ftp)(http)	CEL
GSM21707.DAT.gz	38.5 Mb	(ftp)(http)	DAT