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| Status |
Public on Apr 17, 2004 |
| Title |
Ultraviolet induced gene expression in human basal cell culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This data set was generated as part of Biogen Community Lab's science fair mentorship program. In brief, human basal cells (ATCC: CRL-7761, designation TE 353.Sk) were cultured to 80% confluence. After removing media, cells were treated with a titration of ultraviolet light (Biorad GS GeneLinker, 254 nm energy maximum). Media was then added and cells were grown for 24 hours. Total RNA was harvested using RLT buffer and Qiagen RNeasy kit. 5 ug of total RNA was labeled using standard Affymetrix protocols for Eukaryotic target preparation (GeneChip Expression Analysis Technical Manual Revision 4). GeneChip intensity values were scaled to 2500 RFU.
Keywords = UV
Keywords = ultraviolet
Keywords = basal cell
Keywords: dose response
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| Contributor(s) |
Zou X, Shearstone JR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 15, 2004 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Jeff Shearstone |
| E-mail(s) |
jshearstone@acetylon.com
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| Phone |
617-245-1321
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| Organization name |
Acetylon Pharmaceuticals
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| Department |
Biology
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| Street address |
70 Fargo Street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02210 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA89885 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE1317_RAW.tar |
126.4 Mb |
(http)(custom) |
TAR (of CEL, DAT) |
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