|
| Status |
Public on Sep 02, 2015 |
| Title |
OA_Knee 20 |
| Sample type |
genomic |
| |
|
| Source name |
human knee cartilage from knee osteoarthritis patients
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
scan date: March_2013
kl score: 3
age: 59
sentrixid: 9257626020_R04C02
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from 250mg of ground cartilage using the EZNA DNA/RNA isolation kit (Omega Bio-Tek)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
500ng of DNA was bisulphite converted using the EZ DNA methylation kit (Zymo Research)
|
| |
|
| Hybridization protocol |
Infinium HumanMethylatio450 BeadChip array
|
| Scan protocol |
standard illumina scanner
|
| Description |
All compartments
|
| Data processing |
Minfi Bioconductor Package
|
| |
|
| Submission date |
Sep 02, 2015 |
| Last update date |
Sep 16, 2015 |
| Contact name |
Andrew James Skelton |
| E-mail(s) |
andrew.skelton@ncl.ac.uk
|
| Organization name |
Newcastle University
|
| Department |
Bioinformatics
|
| Lab |
Musculoskeletal Research Group
|
| Street address |
Framlington Place
|
| City |
Newcastle upon Tyne |
| State/province |
Tyne and Wear |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE63695 |
Characterization of the cartilage DNA methylome in knee and hip osteoarthritis. |
|
