|
Status |
Public on Sep 02, 2015 |
Title |
OA_Knee 1 |
Sample type |
genomic |
|
|
Source name |
human knee cartilage from knee osteoarthritis patients
|
Organism |
Homo sapiens |
Characteristics |
gender: Female scan date: March_2013 kl score: 3 age: 62 sentrixid: 9257626014_R05C02
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from 250mg of ground cartilage using the EZNA DNA/RNA isolation kit (Omega Bio-Tek)
|
Label |
Cy5 and Cy3
|
Label protocol |
500ng of DNA was bisulphite converted using the EZ DNA methylation kit (Zymo Research)
|
|
|
Hybridization protocol |
Infinium HumanMethylatio450 BeadChip array
|
Scan protocol |
standard illumina scanner
|
Description |
Mainly patellofemoral
|
Data processing |
Minfi Bioconductor Package
|
|
|
Submission date |
Sep 02, 2015 |
Last update date |
Sep 16, 2015 |
Contact name |
Andrew James Skelton |
E-mail(s) |
andrew.skelton@ncl.ac.uk
|
Organization name |
Newcastle University
|
Department |
Bioinformatics
|
Lab |
Musculoskeletal Research Group
|
Street address |
Framlington Place
|
City |
Newcastle upon Tyne |
State/province |
Tyne and Wear |
ZIP/Postal code |
NE2 4HH |
Country |
United Kingdom |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE63695 |
Characterization of the cartilage DNA methylome in knee and hip osteoarthritis. |
|