|
| Status |
Public on Jul 01, 2007 |
| Title |
placenta-0460-inflamed-infected-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
placenta with chronic malaria
|
| Organism |
Homo sapiens |
| Characteristics |
Parity:Nulliparous
StudyID: 0460
Inflammation: Yes
ChronicPM: case
PM Status: infected
%parasitemia: 0.6
InfantSex: Male
MaternalAge: 19
Birthweight(kg): 2.8
|
| Treatment protocol |
Tissue collected fresh at delivery, stored in RNAlater -80 degrees celsius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Qiagen Rneasy kits per manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr. The chips were then washed and stained with streptavidin-phycoerythrin using the Affymetrix GeneChip System 400.
|
| Scan protocol |
Chips were scanned using the GeneChip Scanner.
|
| Description |
Gene expression data from a placenta with chronic malaria
|
| Data processing |
Affymetrix .CEL files were normalized using GC-RMA on Acuity 4.0
|
| |
|
| Submission date |
Apr 23, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Atis Muehlenbachs |
| E-mail(s) |
amuehlen@u.washington.edu
|
| Organization name |
Seattle Biomedical Research Institute
|
| Street address |
307 Westlake Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7586 |
Genome wide analysis of placental malaria |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
