|
| Status |
Public on Apr 02, 2015 |
| Title |
H2LNex |
| Sample type |
RNA |
| |
|
| Source name |
extracellular vesicles from breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231-D3H2LN
collected from cell line: extracellular vesicles
|
| Treatment protocol |
miRNA expression in extracellular vesicles was collected from MDA-MB-231-D3H1, MDA-MB-231-D3H2LN, BMD2a and BMD2b breast cancer cell lines.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the QIAzol reagent and the miRNeasy Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA was used by Agilent’s miRNA Complete Labeling and Hybridization Kit
|
| |
|
| Hybridization protocol |
Agilent miRNA expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies Scanner G2505C at 3 micron resolution.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters (protocol miRNA_107_Sep09 and Grid: 046064_D_F_20121223) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Nov 19, 2014 |
| Last update date |
Apr 02, 2015 |
| Contact name |
Naoomi Tominaga |
| Organization name |
National Cancer Center Research institute
|
| Street address |
5-1-1 Tsukiji, Chuo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18044 |
| Series (1) |
| GSE63445 |
miRNA expression of extracellular vesicles |
|
