GEO help: Mouse over screen elements for information.
Status
Public on Mar 21, 2004
Title
267B1 vs. PC3M. Array 1.
Sample type
genomic
Channel 1 Source name
prostate cell line 267B1 DNA, Cy5
Organism
Homo sapiens Extracted molecule
genomic DNA
Channel 2 Source name
prostate cell line PC3M DNA, Cy3
Organism
Homo sapiens Extracted molecule
genomic DNA
Description
The methylation status of 267B1 and PC3M are compared. DNA samples were digested with methylation sensitive enzyme HpaII, ligated with adaptor, PCR amplified and labeled with Cye Dye. The labeled target are hybridized with human promoter array. In general, methylated sequences will not be cut and will not be amplified. Only unmethylated sequences will be amplified and hybridized to arrays.
Submission date
Dec 30, 2003
Last update date
May 28, 2005
Contact name
Yipeng Wang
E-mail(s)
ywang@sdibr.org Phone
8585813960
Organization name
Vaccine Research Institute of San Diego
Street address
City
San Diego
State/province
CA
ZIP/Postal code
92121
Country
USA
Platform ID
GPL864 Series (1)
GSE951 267B1, PC3M, LNCaP Methylation Profile vs. mRNA Expression Level
Data table header descriptions ID_REF
CH1_RAW channel 1 intensity
CH1_BKD_RAW ch1 background
CH2_RAW channel 2 intensity
CH2_BKD_RAW ch2 background
VALUE calculation
Data table ID_REF CH1_RAW CH1_BKD_RAW CH2_RAW CH2_BKD_RAW VALUE 1
172.389999
0
386.950012
0
-0.52747567
2
171.940002
0
315.600006
12.966666
-0.160626526
3
248.559998
0
301.600006
0
0.351994479
4
5110.569824
0
7726.339844
0
0.24422102
5
598.380005
0
777.909973
0
0.185659422
6
175.729996
0
267.640015
1.833333
0.064517787
7
512.789978
0
913.75
3.766667
-0.26302435
8
206.339996
0
289.920013
5.633333
0.185148014
9
343.619995
0
524.049988
0
-0.046027441
10
277.649994
0
346.380005
0
0.294873285
11
205.210007
0
364.51001
0
-0.197668846
12
807.380005
0
1854.540039
0
-0.454315612
13
704.429993
0
1738.02002
0
-0.591964659
14
655.140015
0
805.23999
0
0.279320503
15
705.76001
0
922.369995
0
0.21515711
16
440.450012
0
654.969971
0
-0.025298795
17
375.390015
0
661.390015
0
-0.270834303
18
598.72998
0
981.640015
0
-0.124840844
19
193.550003
0
329.820007
0
-0.127041138
20
217.929993
2.2
352.859985
0
-0.07989036
17328 796 Kbytes .Supplementary data files not provided
