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Sample GSM1450483 Query DataSets for GSM1450483
Status Public on Nov 30, 2014
Title TU_BRCA1_3 [aCGH]
Sample type genomic
 
Channel 1
Source name human DNA reference pool
Organism Homo sapiens
Characteristics sample type: self-made DNA pool, from blood samples of ten healthy female donors
Extracted molecule genomic DNA
Extraction protocol DNA was extracted by Proteinase K treatment with subsequent phenol-chloroform extraction
Label Cy5-dUTP
Label protocol Standard Agilent Genomic DNA labeling kit Plus Protocol without enzymatic restriction
 
Channel 2
Source name PrimaryTumorTissue_BRCA1
Organism Homo sapiens
Characteristics tissue: Tumor
sample type: fresh-frozen
subtype: BRCA1-mutated tumor
Extracted molecule genomic DNA
Extraction protocol DNA was extracted by Proteinase K treatment with subsequent phenol-chloroform extraction
Label Cy3-dUTP
Label protocol Standard Agilent Genomic DNA labeling kit Plus Protocol without enzymatic restriction
 
 
Hybridization protocol Standard Agilent Protocol
Scan protocol Arrays were scanned using Agilent microarray scanner according to the manufacturer´s instructions
Data processing For image analysis, default CGH settings of the Feature Extraction software (Agilent) were applied. Output files of the Feature Extraction were further investigated by Agilent´s CHG data analysis software, Genomic-Workbench.
algorithm ADM2
 
Submission date Jul 30, 2014
Last update date Nov 30, 2014
Contact name Jessica Rößler
Organization name Medical School Hannover
Department Pathology
Street address Carl-Neuberg Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platform ID GPL10123
Series (2)
GSE59902 DNA methylation study in breast cancer [aCGH]
GSE59903 DNA methylation study in breast cancer

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy3/Cy5 (test/reference)

Data table
ID_REF VALUE
1 7.413202188e-002
2 0.000000000e+000
3 0.000000000e+000
4 0.000000000e+000
5 0.000000000e+000
6 0.000000000e+000
7 0.000000000e+000
8 0.000000000e+000
9 0.000000000e+000
10 0.000000000e+000
11 0.000000000e+000
12 0.000000000e+000
13 0.000000000e+000
14 0.000000000e+000
15 0.000000000e+000
16 0.000000000e+000
17 0.000000000e+000
18 0.000000000e+000
19 0.000000000e+000
20 0.000000000e+000

Total number of rows: 180880

Table truncated, full table size 4219 Kbytes.




Supplementary file Size Download File type/resource
GSM1450483_izmp2011_252206026649_S01_CGH_1100_Jul11_1_4.txt.gz 18.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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