|
| Status |
Public on Feb 01, 2007 |
| Title |
Thatcher_Lr34 (resistant), mock inoculated distal leaf, replication 2 |
| Sample type |
RNA |
| |
|
| Source name |
Fully expanded wheat flag leaves, distal half, mock inoculated
|
| Organism |
Triticum aestivum |
| Characteristics |
Genotype: Thatcher_Lr34
Growth stage: mid-anthesis
|
| Treatment protocol |
Plants were sprayed with Soltrol oil in the evening and were then incubated in a mist chamber with 100% RH for 12 hr. The plants were returned to the growth chamber until sampling at 48 hr after removal from the mist chamber.
|
| Growth protocol |
Plants were grown in 4-liter pots in growth chamber (Conviron PGW-36) under 15C night/20C day (+/- 1C) with a 16 hr photoperiod. Pots were fertilized at planting with 5 g/pot 14-14-14 Osmocote (The Scotts Company, Marysville, OH) and thereafter every two weeks with 0.5 g/pot 15-30-15 Miracle-Gro All Purpose Plant Food (Scott's Miracle-Gro Products, Inc., Marysville, OH) and 0.1 ml/pot Fertilome Chelated Liquid Iron and Other Micronutrients (Voluntary Purchasing Groups, Inc., Bonham, TX).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip wheat Genome Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Wheat fully expanded flag leaf, mock inoculated distal half, biological replication 2.
|
| Data processing |
The data were analyzed with Affymetrix GCOS software using default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Nov 02, 2006 |
| Last update date |
Nov 08, 2006 |
| Contact name |
Jianfa Bai |
| E-mail(s) |
jbai@ksu.edu
|
| Phone |
785-532-4332
|
| Organization name |
Kansas State University
|
| Department |
Diagnostic Medicine and Pathobiology
|
| Street address |
1800 Denison Ave
|
| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506-5502 |
| Country |
USA |
| |
|
| Platform ID |
GPL3802 |
| Series (1) |
| GSE6227 |
Expression data from rust or mock inoculated, fully expanded flag leaf halves |
|
