|
| Status |
Public on Sep 10, 2014 |
| Title |
PRT_25_PRE |
| Sample type |
RNA |
| |
|
| Source name |
VASTUS LATERALIS
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Vastus Lateralis
sample id: 25
exercise type (aerobic or resistance): RESISTANCE
pre exercise/ post exercise: PRE
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A biopsy was collected from the right Vastus Lateralis under local anaesthesia and RNA was isolated from muscle (~10 mg) using mirVana™ Kit , Low molecular weight RNA was biotin-labeled with FlashTag™ Biotin HSR RNA Labeling Kit
|
| Label |
biotin
|
| Label protocol |
low molecular weight RNA was biotin-labeled with FlashTag™ Biotin HSR RNA Labeling Kit
|
| |
|
| Hybridization protocol |
21.5 µl of high-quality biotin-labeled miRNA sample was hybridized to Affymetrix Gene-Chip® miRNA 2.0 Array for 16 h according to Affymetrix protocol
|
| Scan protocol |
The arrays were washed and stained on the Affymetrix Fluidics station 400 and scanned with a Hewlett Packard G2500A gene Array Scanner
|
| Data processing |
Affymetrix® miRNA QC Tool v 1.1.1.0 was used for the data summarization, normalization, and microarray quality control
|
| |
|
| Submission date |
Jun 05, 2014 |
| Last update date |
Sep 10, 2014 |
| Contact name |
David Rowlands |
| E-mail(s) |
D.S.Rowlands@massey.ac.nz
|
| Organization name |
Massey University
|
| Department |
Institute of Food, Nutrition and Human Health
|
| Lab |
Dr. David Rowlands
|
| Street address |
63 Wallace St Mt Cook
|
| City |
Wellington |
| State/province |
Wellington |
| ZIP/Postal code |
6011 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE58248 |
Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [miRNA data] |
| GSE58250 |
Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity |
|
