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| Status |
Public on Sep 10, 2014 |
| Title |
Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [miRNA data] |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Analysis of skeletal muscle miRNA expression from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic ecercise and the other resistance training exercise)
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| Overall design |
A biopsy was collected from the right Vastus Lateralis under local anaesthesia and RNA was isolated from muscle (~10 mg) using mirVana™ Kit , Low molecular weight RNA was biotin-labeled with FlashTag™ Biotin HSR RNA Labeling Kit. 21.5 µl of high-quality biotin-labeled miRNA sample was hybridized to Affymetrix Gene-Chip® miRNA 2.0, stained on Affymetrix Fluidics station 400 and scanned with a Hewlett Packard G2500A gene Array Scanner. Affymetrix® miRNA QC Tool v 1.1.1.0 was used for the data summarization, normalization, and microarray quality control.
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| Contributor(s) |
Rowlands D, Giri M |
| Citation(s) |
25138607 |
| Submission date |
Jun 05, 2014 |
| Last update date |
May 02, 2017 |
| Contact name |
David Rowlands |
| E-mail(s) |
D.S.Rowlands@massey.ac.nz
|
| Organization name |
Massey University
|
| Department |
Institute of Food, Nutrition and Human Health
|
| Lab |
Dr. David Rowlands
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| Street address |
63 Wallace St Mt Cook
|
| City |
Wellington |
| State/province |
Wellington |
| ZIP/Postal code |
6011 |
| Country |
New Zealand |
| |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE58250 |
Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity |
|
| Relations |
| BioProject |
PRJNA251732 |
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