|
Status |
Public on Feb 27, 2014 |
Title |
380b |
Sample type |
SRA |
|
|
Source name |
Whole blood
|
Organism |
Homo sapiens |
Characteristics |
time point: post-infection tissue: whole blood infectious agent: Plasmodium falciparum (Pf) ID: 380 class: Delayed Fever age: 7.671232877 gender: Male anemia status at baseline: 1 s. haematobium at baseline: 0 pf infection status: I parasite density: 265.2519 temp max: 36.7 extraction date: 2/20/13 10:30 sequencing date: 21-Oct-13
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from whole blood, and depleted of rRNA and globin RNA (ScriptSeqâ„¢ Complete Gold Kit:Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
Sample 178
|
Data processing |
Illumina Casava1.8.2 software used for basecalling. Sequenced reads were mapped to GRCh3 using tophat v2.0.8 FPKM values were obtained from the tophat output Genome_build: GRCh37 Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for genes and transcript isoforms, and read counts per gene.
|
|
|
Submission date |
Feb 26, 2014 |
Last update date |
Aug 26, 2019 |
Contact name |
Ravi Sanka |
E-mail(s) |
rsanka@jcvi.org
|
Organization name |
JCVI
|
Street address |
9704 Medical Center Drive
|
City |
Rockville |
State/province |
Maryland |
ZIP/Postal code |
20850 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE52166 |
Molecular Hallmarks of Naturally Acquired Immunity to Malaria |
|
Relations |
BioSample |
SAMN02665972 |
SRA |
SRX476847 |