|
| Status |
Public on Jun 03, 2015 |
| Title |
Wuhan1, 2 d after water inoculation, rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Water-inoculated Wuhan1 spikelets_2 d
|
| Organism |
Triticum aestivum |
| Characteristics |
cultivar: spring wheat cv Wuhan1
tissue: inoculated spikelets
tissue age: heads after mid-anthesis
inoculated with: Water
day after inoculation: 2
|
| Treatment protocol |
Wheat heads at mid-anthesis were inoculated as follow: 10 µl of a 100,000 spores/ml suspension of the isolate Fusarium graminearum strain DAOM 180378 was point-inoculated into each of two basal florets of each developed spikelet of the wheat heads. Water was used as a mock-inoculation treatment. The inoculated plants were mist-irrigated for 10 second intervals every 15 minutes for 48 hours to maintain high humidity at a temperature of 25 oC. Five treated heads were used for each biological replicate, and inoculated spikelets were sampled at 1, 2 and 4 days after inoculation. The sampled tissues were immediately frozen in liquid nitrogen and stored at -80 C until used.
|
| Growth protocol |
Plants were grown in growth cabinets at 20 C/16 hrs daylight; 16 C/8 hrs night until heads reach mid anthesis; then they were moved to the misting room.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each sample was isolated using a modified form of the guanidine isothiocyanate-cesium chloride method of Chirgwin et al (Biochem. 18: 5294-5299, 1979), as outlined in Ohan and Heikkila (Dev. Genet. 17: 176-184, 1995). The RNA samples were treated with an RNase-free DNase (Qiagen, Mississauga, Canada) and cleaned up using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix instructions for the GeneChip® HT One-Cycle Target Labeling and Controls Kit. All samples were processed in one experimental batch.
|
| |
|
| Hybridization protocol |
According to Affymetrix instructions for the GeneChip® HT One-Cycle Target Labeling and Controls Kit. All samples were processed in one experimental batch.
|
| Scan protocol |
According to Affymetrix instructions for the GeneChip® HT One-Cycle Target Labeling and Controls Kit, using the Affymetrix GeneChip® 3000 Scanner.
|
| Description |
WhH2 rep2
Gene expression data from water-inoculated Wuhan1 spikelets , 2 d after inoculation
|
| Data processing |
The probe-level data for the whole profiling experiment were assembled into one dataset using the Robust Multichip Analysis (Irizarry et al, 2002) implemented in the software Acuity 4.0 (Molecular Devices, CA, USA), with quantile normalization and summarization by median polish.
|
| |
|
| Submission date |
Jan 30, 2014 |
| Last update date |
Jun 04, 2015 |
| Contact name |
Thérèse Ouellet |
| E-mail(s) |
therese.ouellet@canada.ca
|
| Phone |
613-759-1658
|
| Organization name |
Agriculture and Agri-Food Canada
|
| Department |
Eastern Cereal and Oilseed Research Centre
|
| Street address |
960 Carling Ave, room 2091
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A 0C6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3802 |
| Series (2) |
| GSE54553 |
Gene expression during early infection of the resistant spring wheat cultivar Wuhan1 with Fusarium graminearum, the major causal agent of fusarium head blight in wheat |
| GSE54556 |
Gene expression during early infection by Fusarium graminearum in wheat lines susceptible and resistant to fusarium head blight |
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