|
| Status |
Public on Sep 21, 2014 |
| Title |
Control shRNA for GFP-P19 cells without neuronal differentiation, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Control GFP shRNA_P19_w/x neuronal differentiation
|
| Organism |
Mus musculus |
| Characteristics |
cell line: P19
cell type: embryonal carcinoma cells
transfected with: Control GFP shRNA
treated with: none
|
| Treatment protocol |
Neuronal differentiation by retinoic acid treatment
|
| Growth protocol |
P19 mouse embryonal carcinoma cells were purchased from ATCC and maintained in MEMa containing 7.5% calf serum and 2.5% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
microRNAs were labeled using the flashtag biotin assay as per the manufacturer's (Genisphere)directions. In brief, the process begins with a brief tailing reaction followed by ligation of the biotinylated signal molecule to the target RNA sample.
|
| |
|
| Hybridization protocol |
Hybridization of the affymetrix microRNA arrays was done overnight at 45C for 16 hours in a hybridization oven.
|
| Scan protocol |
Scanning of the washed, stained arrays was done using Affymetrix 3000 7G scanner
|
| Description |
RMA-PI9un_1_2.CEL
|
| Data processing |
the image data was analyzed using the miRNA QC tool software for quality control. The raw data was preprocessed by Affymetrix miRNA QC Tool (Version 1.1.1.0) with the workflow of global background correction, quantile normalization and median polish summarization (miRNA-2_0.cdf).
|
| |
|
| Submission date |
Nov 14, 2013 |
| Last update date |
Sep 21, 2014 |
| Contact name |
Yanqin Yang |
| E-mail(s) |
yanqin.yang@nih.gov
|
| Organization name |
NIH
|
| Street address |
10 Center Dr.
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE52377 |
Differential expression of mature miRNAs in P19 cells during neuronal differentiation and by Rbfox3 depletion. |
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