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| Status |
Public on Sep 21, 2014 |
| Title |
Differential expression of mature miRNAs in P19 cells during neuronal differentiation and by Rbfox3 depletion. |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To investigate whether Rbfox3 could alter the expression level of miRNAs during neuronal differentiation of P19 cells, we performed miRNA microarray analysis using the RNAs extracted from untreated (undifferentiated) P19-GFP, RA-treated (neuronally differentiated) P19-GFP, or RA-treated P19-T2 cells.
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| Overall design |
Total 9 samples were analyzed. We compared expression levels of P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify miRNAs which had changes in expression levels with p < 0.01. From this miRNA list, we compared among P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify the miRNAs which appeared to correlate with Rbfox3 expression.
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| Contributor(s) |
kim K, Yang Y, Zhu J, Adelstein RS, Kawamoto S |
| Citation(s) |
25240799 |
| Submission date |
Nov 14, 2013 |
| Last update date |
May 02, 2017 |
| Contact name |
Yanqin Yang |
| E-mail(s) |
yanqin.yang@nih.gov
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| Organization name |
NIH
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| Street address |
10 Center Dr.
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| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (9)
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| GSM1264269 |
Control shRNA for GFP-P19 cells without neuronal differentiation, biological rep1 |
| GSM1264270 |
Control shRNA for GFP-P19 cells without neuronal differentiation, biological rep2 |
| GSM1264271 |
Control shRNA for GFP-P19 cells without neuronal differentiation, biological rep3 |
| GSM1264272 |
Control shRNA for GFP-P19 cells with neuronal differentiation, biological rep1 |
| GSM1264273 |
Control shRNA for GFP-P19 cells with neuronal differentiation, biological rep2 |
| GSM1264274 |
Control shRNA for GFP-P19 cells with neuronal differentiation, biological rep3 |
| GSM1264275 |
Rbfox3 shRNA for T2-P19 cells with neuronal differentiation, biological rep1 |
| GSM1264276 |
Rbfox3 shRNA for T2-P19 cells with neuronal differentiation, biological rep2 |
| GSM1264277 |
Rbfox3 shRNA for T2-P19 cells with neuronal differentiation, biological rep3 |
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| Relations |
| BioProject |
PRJNA227739 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52377_RAW.tar |
5.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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