|
| Status |
Public on Oct 01, 2014 |
| Title |
tCNT4h.0h [A25 2-1+7] |
| Sample type |
RNA |
| |
|
| Source name |
tangled carbon nanotubes 4h.0h
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
mice: Female
strain: C57BL/6
time point: 4 h exposure
|
| Treatment protocol |
Nanomaterials were aerosolized with a fluidized bed aerosol generator, Mice were placed inside the exposure chamber and moved back to animal room after 4 h exposure. The animals were sacrified immediately after exposure or after 24h.
|
| Growth protocol |
Female C57BL/6 mice (7-8 weeks old) were housed in groups of four in stainless steel cages, bedded with aspen chip and were provided with standard mouse chow diet and tab water ad lib. 12 h dark/light cycle was used in the animal room temperature being 20-21 degree celcius and humidity of 40-45 %.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation from the lung samples was done with TRIsure and the quantity and purity was determined by NanoDrop spectrophotometer. Further quality check of the total RNA was performed with the Agilent BioAnalyzer, according to the manufacturer's recommendations.
|
| Label |
Cy3
|
| Label protocol |
Samples were amplified using T7 RNA polymerase method and the cRNAs were further labeled with Cy3 and Cy5 dye, according to the manufacturer's recommendations.
|
| |
|
| Hybridization protocol |
Labeled cRNAs were hybridized to Agilent 2-color 60-mer oligo arrays, , according to the manufacturer's recommendations.
|
| Scan protocol |
Slides were washed and scanned with Agilent Microarray scanner G2505C, according to the manufacturer's recommendations.
|
| Description |
Pool3+Pool4_US23502401_252800516533_S01_GE2_1100_Jul11_1_2.txt
|
| Data processing |
Raw intensity values were obtained from Agilent Feature Extraction software and quality checked according to Agilent standard procedures. The median foreground intensities were imported into R software and analyzed with BioConductor package Limma.
|
| |
|
| Submission date |
Aug 25, 2013 |
| Last update date |
Oct 01, 2014 |
| Contact name |
Dario Greco |
| E-mail(s) |
dario.greco@tuni.fi
|
| Organization name |
Tampere University
|
| Department |
Faculty of Medicine and Health Technology
|
| Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
|
| Street address |
Arvo ylpön Katu 34
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE50176 |
Inhalation of rod-like carbon nanotubes causes unconventional asthma |
|
