|
| Status |
Public on Dec 31, 2013 |
| Title |
100µM VX 1hr neuron replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Neuron 100uM VX treatment for 1hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC-deived Neuron
treatment: 100µM VX
time: 1hr
|
| Biomaterial provider |
Cellular Dynamics (Madison, WI)
|
| Treatment protocol |
One week after plating, cells were treated with VX or saline control
|
| Growth protocol |
iPSC-derived neurons (Cellular Dynamics, WI) were seeded at a density of 2.0 X10E6 per well in 6-well plates and maintained according to manufucturer's specifications
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Following treatment, cells were gently rinsed five times with hanks buffered salt solution. Total RNA was isolated from exposed and control cells using miRNeasy Mini Kit (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
500ng total RNA was labeled using the RNA biotin labeling flashtag biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA) as per manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Labelled RNA was hybridized for 16 hrs at 48 degrees C on miRNA 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using fluidics script FS450_0003
|
| Scan protocol |
GeneChips were scanned using Affymetrix 7G GeneChip Scanner using Affymetrix GeneChip Command Console Scan Control 3.1.01228 (Affymetrix 2009)
|
| Description |
Cells maintained in humidified incubator at 37 degres C during treatment
|
| Data processing |
Raw data (CEL files) were imported into Partek Genomic suite v6.6 (Partek Incorporated, St. Louis, MO) and normalized using robust multiarray averaging (RMA)
|
| |
|
| Submission date |
Jul 10, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
E Chepchumba K Yego |
| E-mail(s) |
eky1@case.edu
|
| Phone |
216-375-7334
|
| Organization name |
US Army Medical Research Institute of Chemical Defense
|
| Department |
Research
|
| Street address |
3100 Ricketts Point Road
|
| City |
Aberdeen Proving Ground |
| State/province |
MD |
| ZIP/Postal code |
21010 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE48670 |
microRNA (miRNA) expression data from induced pluripotent stem cell (iPSC)-derived neurons exposed to VX |
|
